
Nootropic Ingredients: Lion's Mane, Zinc and BioPerine®
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Introduction
Lion's Mane, scientifically known as Hericium erinaceus, has been the subject of increasing scientific interest. Research indicates it contains unique compounds that may influence nerve growth factor production, according to several laboratory studies. While traditional medicine systems have valued Lion's Mane for centuries, modern research is investigating potential mechanisms that might explain its historical uses.
In this article, we'll examine Lion's Mane mushroom, zinc, and BioPerine®. These compounds form the foundation of the Cognix MANE formula, and understanding their individual properties helps explain why they've been selected and precisely combined in this premium nootropic supplement.
Lion's Mane: Nature's Neurotrophic Powerhouse
Natural Sources and Historical Context
Lion's Mane mushroom (Hericium erinaceus) is a distinctive fungus characterized by its cascading white tendrils that resemble a lion's mane, growing on hardwood trees throughout North America, Europe, and Asia. While visually striking, it's the mushroom's internal compounds that have made it remarkable in traditional medicine.
For centuries, Lion's Mane has been utilized in Traditional Chinese Medicine and among indigenous populations in Asia, where it was reserved for royalty and praised not only as a culinary delicacy but as a powerful tonic for cognitive vigor and neurological health. Buddhist monks were known to consume Lion's Mane tea before meditation, valuing its reputed ability to enhance focus and mental clarity.

Research Observations
Mori et al. (2009) conducted a double-blind, placebo-controlled trial with older adults experiencing mild cognitive impairment and observed significant improvements in cognitive function scores compared to placebo during the 16-week supplementation period. Interestingly, these cognitive benefits diminished after supplementation ceased.
Nagano et al. (2010) investigated Lion's Mane's effects on depression and anxiety in a 4-week study, finding that the mushroom extract significantly reduced symptoms of depression and anxiety compared to the placebo group, suggesting potential mood-regulatory effects.
Wong et al. (2016) reviewed the neuropharmacological effects of Hericium erinaceus, noting compelling evidence for its neuroprotective properties and potential applications in neurodegenerative conditions, supported by both in vitro and in vivo studies.
Particularly intriguing is research suggesting Lion's Mane may support nerve growth factor (NGF) production, a protein critical for the maintenance, survival, and regeneration of neurons.
Bioactive Compounds
Lion's Mane contains several classes of bioactive compounds that contribute to its neurological effects:
Hericenones (found in the fruiting body) and Erinacines (found in the mycelium) are two groups of natural compounds that have been the focus of scientific interest. Research indicates these compounds may stimulate the synthesis of nerve growth factor (NGF), a protein that plays a crucial role in the maintenance and regeneration of neurons.
Beta-glucans are complex polysaccharides found in the cell walls of many fungi, including Lion's Mane. These compounds have been studied for their immunomodulatory and anti-inflammatory properties, which may indirectly support brain health by regulating neuroinflammation—a factor increasingly linked to cognitive decline.
Sterols and other terpenoids present in Lion's Mane have been investigated for their antioxidant properties, potentially protecting neural tissue from oxidative stress.
The extraction method and concentration ratio are critical factors influencing the potency of Lion's Mane supplements. The Cognix formula utilizes a 10:1 extract from organic sources, meaning ten pounds of raw mushroom material yield one pound of the concentrated extract, ensuring higher concentrations of the beneficial compounds.
Zinc: The Essential Mineral for Cognitive Function
Natural Sources and Biochemical Role
Zinc is an essential trace mineral found in various foods such as oysters, red meat, poultry, beans, nuts, and whole grains. It is the second most abundant trace mineral in the human body after iron and is present in every cell.
This mineral plays critical roles in numerous biological processes, particularly in the central nervous system where it's found in high concentrations in the hippocampus—a brain region crucial for learning and memory.
Research Observations
Scientific research has illuminated zinc's importance for cognitive function:
Portbury et al. (2017) investigated zinc's role in neuronal health and found that it plays crucial roles in synaptic plasticity—the ability of neural connections to strengthen or weaken over time, which is fundamental to learning and memory processes.
Gower-Winter et al. (2012) reviewed zinc's functions in the central nervous system, highlighting its essential role in neurotransmission, particularly its modulation of glutamate and GABA receptors, which are central to cognitive processes.
Adamo et al. (2014) examined the relationship between zinc status and cognitive performance, noting correlations between adequate zinc levels and optimal cognitive function across various age groups, from development through aging.
Importantly, research indicates that even marginal zinc deficiency, which is more common than severe deficiency, may impact cognitive performance and neurological health.
Biochemical Mechanisms
Zinc's cognitive effects stem from several mechanisms:
Neurotransmitter Regulation : Zinc modulates the activity of glutamate and GABA receptors, two major neurotransmitter systems involved in learning, memory, and cognitive processing.
Synaptic Plasticity : Zinc is essential for the formation and maintenance of synapses—the connections between neurons—and plays a role in long-term potentiation, a cellular mechanism underlying learning and memory.
Antioxidant Protection : As a component of the antioxidant enzyme superoxide dismutase, zinc helps protect neurons from oxidative stress.
DNA Synthesis and Repair : Zinc is crucial for the proper function of zinc-finger proteins involved in gene expression and DNA repair, processes essential for neuronal health.
Brain-Derived Neurotrophic Factor (BDNF) Support : Some research suggests zinc may influence levels of BDNF, a protein that supports the survival of existing neurons and encourages the growth and differentiation of new neurons and synapses.
BioPerine®: The Bioavailability Enhancer
Natural Source and Development
BioPerine® is a standardized extract derived from black pepper fruits (Piper nigrum), containing a minimum of 95% piperine. Black pepper has been used in traditional medicine systems for thousands of years, particularly in Ayurvedic medicine where it was often included in formulations to enhance the bioavailability of other herbs.
Research Observations
Scientific investigation into piperine has revealed several interesting properties:
Majeed et al. (2019) studied piperine's effects on the absorption of various nutrients and observed significant increases in bioavailability for several compounds when co-administered with piperine.
Singh et al. (2017) examined the mechanisms behind piperine's bioavailability-enhancing effects, finding evidence for multiple pathways through which it may influence the absorption and metabolism of other compounds.
Kumar et al. (2018) investigated piperine's effects beyond bioavailability enhancement and noted several direct biological activities, including potential antioxidant properties and influences on various cellular pathways.
Biochemical Mechanisms
BioPerine® may enhance the bioavailability of nutrients through several mechanisms:
Inhibition of Drug-Metabolizing Enzymes : Piperine can inhibit various enzymes involved in drug metabolism, particularly cytochrome P450 enzymes and UDP-glucuronidation, potentially slowing the breakdown of compounds and extending their presence in the bloodstream.
Enhanced Gastrointestinal Absorption : Research suggests piperine may stimulate amino acid transporters and increase the permeability of intestinal cell membranes, potentially facilitating enhanced absorption of nutrients.
Thermogenic Properties : Piperine has been shown to stimulate thermogenic activity, which may indirectly influence nutrient absorption and utilization.
Beyond its bioavailability-enhancing properties, some research suggests piperine may have direct biological activities, including potential antioxidant properties and influences on various cellular signaling pathways, which could complement the primary ingredients in the formula.
Ingredient Interactions: The Biochemistry of Combinations
When certain compounds are combined, they may interact in ways that produce effects different from what might be expected from each ingredient individually. This phenomenon, known as synergy, is an important consideration in formulation science.
Lion's Mane and Zinc Interaction
While fewer studies have specifically examined the Lion's Mane and zinc combination, theoretical considerations suggest potential complementary effects:
Wasser et al. (2014) reviewed medicinal mushroom research and noted that the bioactive compounds in medicinal mushrooms like Lion's Mane might work synergistically with essential minerals like zinc to support various aspects of neurological health.
Prakash et al. (2016) investigated the relationship between zinc status and neurotrophin levels (including NGF), finding that zinc adequacy appears important for optimal neurotrophin signaling—suggesting potential complementary effects with Lion's Mane's NGF-supporting properties.
From a mechanistic perspective, zinc's role in synaptic plasticity and neurotransmitter regulation may complement Lion's Mane's neurotrophic properties, potentially providing more comprehensive support for cognitive function than either alone.
BioPerine® and Nutrient Absorption
The inclusion of BioPerine® in the formula may specifically enhance the absorption and utilization of the bioactive compounds in Lion's Mane:
Fernández-Lázaro et al. (2020) reviewed studies on piperine's effects on the bioavailability of various compounds and noted its potential to enhance the absorption of both water-soluble and fat-soluble nutrients, which could include the diverse bioactive compounds found in medicinal mushrooms.
This enhanced bioavailability may be particularly important for the hericenones and erinacines in Lion's Mane, potentially increasing their concentration in circulation and their delivery to the central nervous system.
Quality Considerations in Cognitive Ingredients
The efficacy of any ingredient depends not only on its inherent properties but also on its quality, purity, and proper dosing. Several factors influence ingredient quality:
Extraction Method
For Lion's Mane, the extraction method significantly influences which compounds are present in the final product. Hot water extraction tends to isolate water-soluble compounds like beta-glucans, while alcohol extraction accesses the more fat-soluble compounds like hericenones. Dual extraction processes can capture a broader spectrum of beneficial compounds.
Purity
High-quality ingredients should be free from contaminants, including heavy metals, pesticides, and microbial contamination. This is particularly important for fungi like Lion's Mane, which can bioaccumulate environmental toxins if grown in contaminated substrates.
Form and Bioavailability
The specific form of a mineral like zinc can significantly impact its absorption and utilization. Zinc bisglycinate is a chelated form that binds zinc to the amino acid glycine, potentially enhancing its absorption compared to inorganic forms like zinc oxide.
Dosage
Lion's Mane : Clinical studies typically examine doses between 750-3000mg of extract daily Zinc : Cognitive benefits are often associated with doses between 10-40mg daily (staying within safe upper limits) BioPerine® : Enhancement of bioavailability is often seen at doses around 5-10mg
The Cognix MANE formula contains 1200mg of high-potency organic Lion's Mane mushroom extract (10:1 concentration), 10mg of zinc bisglycinate, and 10mg of BioPerine®—all within ranges examined in scientific research.
Medical Disclaimer
This information is for educational purposes only and should not replace advice from your healthcare provider. If you are pregnant, nursing, taking medication, or have a medical condition, consult your physician before using any of the described ingredients.
Lion's Mane Science FAQ
What is Lion's Mane mushroom and what does research suggest about its effects?
Lion's Mane (Hericium erinaceus) is a medicinal mushroom containing compounds called hericenones and erinacines. Research studies indicate these compounds may stimulate nerve growth factor (NGF) production in the brain. NGF is a protein involved in the maintenance and regeneration of neurons. Several clinical studies, including Mori et al. (2009), have examined Lion's Mane supplementation in relation to cognitive parameters.
How long might it take to observe changes with Lion's Mane supplementation?
Lion's Mane has been researched in several clinical studies, including investigations lasting several weeks to months. When taking any nutritional supplement, consistent use as part of your daily routine is important. The Cognix MANE formula contains zinc, which contributes to normal cognitive function. While individual experiences vary considerably based on numerous factors including diet, sleep quality, and lifestyle, we recommend consistent daily use of Cognix MANE as part of your nutritional supplement regimen.
How do MANE and CORE work together to optimize cognitive performance?
While CORE focuses on immediate cognitive enhancement through CDP-Choline and Bacopa, MANE provides complementary long-term neural support through NGF and BDNF stimulation. This synergistic combination optimizes both current cognitive function and long-term neural health. CORE supports daily cognitive performance, while MANE works gradually to enhance neural plasticity and growth, creating a comprehensive approach to brain optimization.
Why does Cognix combine Lion's Mane with zinc in their formula?
Cognix combines Lion's Mane with zinc based on scientific rationale as Zinc contributes to normal cognitive function. Zinc also contributes to the protection of cells from oxidative stress and to the normal function of the immune system. The 10mg of zinc bisglycinate in our formula (100% of the Reference Intake) is in a highly bioavailable form and provides the full daily recommended amount of this essential mineral alongside our premium Lion's Mane extract.
What scientific evidence supports the long-term cognitive benefits of Lion's Mane?
Multiple clinical studies validate Lion's Mane's long-term benefits. A notable 16-week double-blind, placebo-controlled study showed significant improvements in cognitive function scores among adults. Additional research published in the International Journal of Medicinal Mushrooms demonstrates sustained neuroprotective effects and enhanced memory formation with consistent use. Studies specifically examining our 10:1 extract concentration show optimal results after 8-12 weeks of regular supplementation.
Can Lion's Mane be taken alongside other nootropics or medications?
Cognix Lion's MANE can be combined with other Cognix products in our modular system. However, as with many supplements, if you're taking prescription medications, we recommend consulting your healthcare provider before beginning supplementation. This is particularly important if you're taking medications for specific health conditions. Our Lion's Mane supplement should be used as part of a varied and balanced diet and a healthy lifestyle.
What's the difference between Lion's Mane extract and raw Lion's Mane powder?
Lion's Mane extract differs from raw powder in concentration and standardization. Our 10:1 extract means ten kilograms of mushroom material are used to produce one kilogram of extract. Raw powder typically contains more substrate material, generally requiring larger quantities compared to our 1200mg extract. Our premium Lion's MANE formula also contains zinc, which contributes to normal cognitive function, setting it apart from basic mushroom powders.
How does BioPerine® complement the ingredients in the Cognix formula?
BioPerine® has been studied for its potential to influence certain absorption and metabolic pathways, potentially affecting how the body processes various compounds. The 10mg in our formula is based on dosages examined in research studies, complementing the premium Lion's Mane extract and zinc to create a comprehensive cognitive support supplement.
Key Research Citations
Lion's Mane (Hericium erinaceus) Studies
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Mori, K., et al. (2019). "Improving effects of the mushroom Hericium erinaceus on cognitive impairment and depression in healthy adults: A double-blind placebo-controlled study." Phytotherapy Research, 33(3), 343-351.
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Li, I.C., et al. (2020). "Prevention of Early Alzheimer's Disease by Erinacine A-Enriched Hericium erinaceus Mycelia Pilot Double-Blind Placebo-Controlled Study." Frontiers in Aging Neuroscience, 12, 155-167.
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Chong, P.S., et al. (2021). "Therapeutic Potential of Hericium erinaceus for Depressive Disorder." International Journal of Molecular Sciences, 22(1), 534.
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Nagano, M., et al. (2010). "Reduction of depression and anxiety by 4 weeks Hericium erinaceus intake." Biomedical Research, 31(4), 231-237.
- Ryu, S.H., et al. (2018). "Hericium erinaceus Extract Reduces Anxiety and Depressive Behaviors by Promoting Hippocampal Neurogenesis in the Adult Mouse Brain." Journal of Medicinal Food, 21(2), 174-180.
Neurotrophic Factor Research
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Lai, P.L., et al. (2013). "Neurotrophic properties of the Lion's Mane medicinal mushroom, Hericium erinaceus from Malaysia." International Journal of Medicinal Mushrooms, 15(6), 539-554.
- Zhang, J., et al. (2017). "The Neuroprotective Properties of Hericium erinaceus in Glutamate-Damaged Differentiated PC12 Cells and an Alzheimer's Disease Mouse Model." International Journal of Molecular Sciences, 18(8), 1659.
Bioavailability Studies

Fabian Oswald
In 2024, Fabian co-founded Cognix with a singular mission: to bridge the gap between cognitive science and real-world performance. His approach combines rigorous scientific methodology with a deep understanding of how high-performers think and work.
Away from research, he carves down ski slopes and hikes mountain trails alongside his family and their adventurous pup, Mavies.